Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish

Due to its genetic amenability coupled with advances in genome editing, zebrafish is an excellent model to examine the function of (epi)genomic elements. Here, we repurposed the Ac/Ds maize transposition system to efficiently characterise zebrafish cis-regulated elements, also known as enhancers, in...

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Autores principales: Chong-Morrison, Vanessa, Mayes, Sarah, Simões, Filipa C., Senanayake, Upeka, Carroll, Dervla S., Riley, Paul R., Wilson, Stephen W., Sauka-Spengler, Tatjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Methods & Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320716/
https://www.ncbi.nlm.nih.gov/pubmed/37367831
http://dx.doi.org/10.1242/bio.059995
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author Chong-Morrison, Vanessa
Mayes, Sarah
Simões, Filipa C.
Senanayake, Upeka
Carroll, Dervla S.
Riley, Paul R.
Wilson, Stephen W.
Sauka-Spengler, Tatjana
author_facet Chong-Morrison, Vanessa
Mayes, Sarah
Simões, Filipa C.
Senanayake, Upeka
Carroll, Dervla S.
Riley, Paul R.
Wilson, Stephen W.
Sauka-Spengler, Tatjana
author_sort Chong-Morrison, Vanessa
collection PubMed
description Due to its genetic amenability coupled with advances in genome editing, zebrafish is an excellent model to examine the function of (epi)genomic elements. Here, we repurposed the Ac/Ds maize transposition system to efficiently characterise zebrafish cis-regulated elements, also known as enhancers, in F0-microinjected embryos. We further used the system to stably express guide RNAs enabling CRISPR/dCas9-interference (CRISPRi) perturbation of enhancers without disrupting the underlying genetic sequence. In addition, we probed the phenomenon of antisense transcription at two neural crest gene loci. Our study highlights the utility of Ac/Ds transposition as a new tool for transient epigenome modulation in zebrafish.
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spelling pubmed-103207162023-07-06 Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish Chong-Morrison, Vanessa Mayes, Sarah Simões, Filipa C. Senanayake, Upeka Carroll, Dervla S. Riley, Paul R. Wilson, Stephen W. Sauka-Spengler, Tatjana Biol Open Methods & Techniques Due to its genetic amenability coupled with advances in genome editing, zebrafish is an excellent model to examine the function of (epi)genomic elements. Here, we repurposed the Ac/Ds maize transposition system to efficiently characterise zebrafish cis-regulated elements, also known as enhancers, in F0-microinjected embryos. We further used the system to stably express guide RNAs enabling CRISPR/dCas9-interference (CRISPRi) perturbation of enhancers without disrupting the underlying genetic sequence. In addition, we probed the phenomenon of antisense transcription at two neural crest gene loci. Our study highlights the utility of Ac/Ds transposition as a new tool for transient epigenome modulation in zebrafish. The Company of Biologists Ltd 2023-06-27 /pmc/articles/PMC10320716/ /pubmed/37367831 http://dx.doi.org/10.1242/bio.059995 Text en © 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Methods & Techniques
Chong-Morrison, Vanessa
Mayes, Sarah
Simões, Filipa C.
Senanayake, Upeka
Carroll, Dervla S.
Riley, Paul R.
Wilson, Stephen W.
Sauka-Spengler, Tatjana
Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish
title Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish
title_full Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish
title_fullStr Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish
title_full_unstemmed Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish
title_short Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish
title_sort ac/ds transposition for crispr/dcas9-sid4x epigenome modulation in zebrafish
topic Methods & Techniques
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320716/
https://www.ncbi.nlm.nih.gov/pubmed/37367831
http://dx.doi.org/10.1242/bio.059995
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