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Protocol to assess substrate dephosphorylation by serine/threonine phosphoprotein phosphatases in vitro

Serine/threonine protein phosphatase 2 (PP2A) forms heterotrimeric holoenzymes, where a scaffold subunit bridges the PP2A catalytic subunit to a B regulatory subunit, e.g., B55α. The PP2A/B55α holoenzyme plays key roles in signaling and cell-cycle control targeting multiple substrates. Here, we desc...

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Detalles Bibliográficos
Autores principales: Wasserman, Jason S., Feiser, Felicity, Palacio, Seren, Patel, Kishan, Gonzalez, Joy, Fowle, Holly, Graña, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10322857/
https://www.ncbi.nlm.nih.gov/pubmed/37074907
http://dx.doi.org/10.1016/j.xpro.2023.102148