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Protocol for in vivo and in vitro study of lymphatic valve formation driven by shear stress signaling pathway
Here we detail a protocol for the isolation and processing of lymphatic enriched tissue of mouse models for the purpose of immunostaining and quantification of lymphatic valves, vessel length, and vessel diameter. Furthermore, we describe an optimized protocol for exposing treated human dermal lymph...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10322886/ https://www.ncbi.nlm.nih.gov/pubmed/37071531 http://dx.doi.org/10.1016/j.xpro.2023.102141 |