Protocol for generation of multicellular spheroids through reduced gravity

Multicellular spheroids are useful models for drug testing or studying tumor biology, but their production requires specialized approaches. Here, we present a protocol to produce viable spheroids by slow rotation around a horizontal axis using standard culture tubes. We describe steps for both seed...

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Detalles Bibliográficos
Autores principales: Zinn, Dylan A., Mehner, Christine, Patel, Tushar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323117/
https://www.ncbi.nlm.nih.gov/pubmed/37133991
http://dx.doi.org/10.1016/j.xpro.2023.102264

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323117/
https://www.ncbi.nlm.nih.gov/pubmed/37133991
http://dx.doi.org/10.1016/j.xpro.2023.102264

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