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Protocol for generation of multicellular spheroids through reduced gravity
Multicellular spheroids are useful models for drug testing or studying tumor biology, but their production requires specialized approaches. Here, we present a protocol to produce viable spheroids by slow rotation around a horizontal axis using standard culture tubes. We describe steps for both seed...
Autores principales: | Zinn, Dylan A., Mehner, Christine, Patel, Tushar |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323117/ https://www.ncbi.nlm.nih.gov/pubmed/37133991 http://dx.doi.org/10.1016/j.xpro.2023.102264 |
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