Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures

Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in intact colorectal cancer organoids derived...

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Detalles Bibliográficos
Autores principales: Martinez-Ordoñez, Anxo, Cid-Diaz, Tania, Duran, Angeles, Han, Qixiu, Moscat, Jorge, Diaz-Meco, Maria T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323124/
https://www.ncbi.nlm.nih.gov/pubmed/37083323
http://dx.doi.org/10.1016/j.xpro.2023.102243

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323124/
https://www.ncbi.nlm.nih.gov/pubmed/37083323
http://dx.doi.org/10.1016/j.xpro.2023.102243

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