Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures

Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in intact colorectal cancer organoids derived...

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Detalles Bibliográficos
Autores principales: Martinez-Ordoñez, Anxo, Cid-Diaz, Tania, Duran, Angeles, Han, Qixiu, Moscat, Jorge, Diaz-Meco, Maria T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323124/
https://www.ncbi.nlm.nih.gov/pubmed/37083323
http://dx.doi.org/10.1016/j.xpro.2023.102243
Descripción
Sumario:Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in intact colorectal cancer organoids derived from mice. We also describe additional steps for co-culture with mouse fibroblasts to enable the study of interactions with other cellular components of the tissue microenvironment. For complete details on the use and execution of this protocol, please refer to Martinez-Ordoñez et al. (2023).(1)

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