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Investigating pioneer factor activity and its coordination with chromatin remodelers using integrated synthetic oligo assay

Chromatin accessibility is regulated by pioneer factors (PFs) and chromatin remodelers (CRs). Here, we present a protocol, based on integrated synthetic oligonucleotide libraries in yeast, to systematically interrogate the nucleosome-displacing activities of PFs and their coordination with CRs. We d...

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Detalles Bibliográficos
Autores principales: Chen, Hengye, Yan, Chao, Dhasarathy, Archana, Kladde, Michael, Bai, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323128/
https://www.ncbi.nlm.nih.gov/pubmed/37289591
http://dx.doi.org/10.1016/j.xpro.2023.102279