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Investigating pioneer factor activity and its coordination with chromatin remodelers using integrated synthetic oligo assay
Chromatin accessibility is regulated by pioneer factors (PFs) and chromatin remodelers (CRs). Here, we present a protocol, based on integrated synthetic oligonucleotide libraries in yeast, to systematically interrogate the nucleosome-displacing activities of PFs and their coordination with CRs. We d...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323128/ https://www.ncbi.nlm.nih.gov/pubmed/37289591 http://dx.doi.org/10.1016/j.xpro.2023.102279 |