Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans

We present an optimized protocol for in vivo affinity purification proteomics and biochemistry using the model organism C. elegans. We describe steps for target tagging, large-scale culture, affinity purification using a cryomill, mass spectrometry and validation of candidate binding proteins. Our a...

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Detalles Bibliográficos
Autores principales: Desbois, Muriel, Pak, Joseph S., Opperman, Karla J., Giles, Andrew C., Grill, Brock
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323129/
https://www.ncbi.nlm.nih.gov/pubmed/37294631
http://dx.doi.org/10.1016/j.xpro.2023.102262

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323129/
https://www.ncbi.nlm.nih.gov/pubmed/37294631
http://dx.doi.org/10.1016/j.xpro.2023.102262

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