Cargando…

Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans

We present an optimized protocol for in vivo affinity purification proteomics and biochemistry using the model organism C. elegans. We describe steps for target tagging, large-scale culture, affinity purification using a cryomill, mass spectrometry and validation of candidate binding proteins. Our a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Desbois, Muriel, Pak, Joseph S., Opperman, Karla J., Giles, Andrew C., Grill, Brock
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323129/ https://www.ncbi.nlm.nih.gov/pubmed/37294631 http://dx.doi.org/10.1016/j.xpro.2023.102262

Ejemplares similares

Developmental Function of the PHR Protein RPM-1 Is Required for Learning in Caenorhabditis elegans
por: Giles, Andrew C., et al.
Publicado: (2015)

Ubiquitin ligase activity inhibits Cdk5 to control axon termination
por: Desbois, Muriel, et al.
Publicado: (2022)

Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans
por: Ahier, Arnaud, et al.
Publicado: (2021)

Autophagy is inhibited by ubiquitin ligase activity in the nervous system
por: Crawley, Oliver, et al.
Publicado: (2019)

O-GlcNAc transferase OGT-1 and the ubiquitin ligase EEL-1 modulate seizure susceptibility in C. elegans
por: Suthakaran, Nirthieca, et al.
Publicado: (2021)

Tandem affinity purification protocol for isolation of protein complexes from Schizosaccharomyces pombe
por: Cipak, Lubos, et al.
Publicado: (2022)

A generic protocol for the affinity-purification of native macromolecular complexes from poxvirus-infected cells
por: Bartuli, Julia, et al.
Publicado: (2022)

Purification of hetero-oligomeric protein variants using a modified tandem affinity purification approach
por: Parui, Aasna L., et al.
Publicado: (2023)

Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification
por: Han, Xingfa, et al.
Publicado: (2021)

Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function
por: Chang, Chao, et al.
Publicado: (2023)

RPM-1 is localized to distinct subcellular compartments and regulates axon length in GABAergic motor neurons
por: Opperman, Karla J, et al.
Publicado: (2014)

Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells
por: Ti, Shih-Chieh, et al.
Publicado: (2020)

Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts
por: Reusch, Sebastian, et al.
Publicado: (2020)

An optimized protocol for isolation of S-nitrosylated proteins from C. elegans
por: Seth, Puneet, et al.
Publicado: (2021)

Protocol for establishing a protein-protein interaction network using tandem affinity purification followed by mass spectrometry in mammalian cells
por: Bian, Weixiang, et al.
Publicado: (2022)

A MIG-15/JNK-1 MAP kinase cascade opposes RPM-1 signaling in synapse formation and learning
por: Crawley, Oliver, et al.
Publicado: (2017)

Purification and quantitative proteomic analysis of cell bodies and protrusions
por: Dermit, Maria, et al.
Publicado: (2021)

Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis
por: Tani, Naoki, et al.
Publicado: (2022)

Differential affinity purification and mass spectrometry analysis of two nuclear pore complex isoforms in yeast S. cerevisiae
por: Bensidoun, Pierre, et al.
Publicado: (2023)

Affinity Purification Strategies for Proteomic Analysis of Transcription Factor Complexes
por: Giambruno, Roberto, et al.
Publicado: (2013)

Optimized protocol for the identification of lipid droplet proteomes using proximity labeling proteomics in cultured human cells
por: Peterson, Clark W.H., et al.
Publicado: (2021)

Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling
por: Emmenegger, Marc, et al.
Publicado: (2023)

Quantification of mRNA ribosomal engagement in human neurons using parallel translating ribosome affinity purification (TRAP) and RNA sequencing
por: Rodrigues, Deivid Carvalho, et al.
Publicado: (2020)

Purification of mouse axoplasmic proteins from dorsal root ganglia nerves for proteomics analysis
por: Kong, Guiping, et al.
Publicado: (2022)

In vivo imaging of a PVD neuron in Caenorhabditis elegans
por: Wang, Xinjian, et al.
Publicado: (2021)

A protocol for human bone marrow adipocyte isolation and purification
por: Attané, Camille, et al.
Publicado: (2021)

Protocol for the purification and transcriptomic analysis of mouse astrocytes using GFAT
por: Labarta-Bajo, Lara, et al.
Publicado: (2023)

Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells
por: Chowdhury, Shanin, et al.
Publicado: (2023)

The Nesprin Family Member ANC-1 Regulates Synapse Formation and Axon Termination by Functioning in a Pathway with RPM-1 and β-Catenin
por: Tulgren, Erik D., et al.
Publicado: (2014)

Histone Purification Combined with High‐Resolution Mass Spectrometry to Examine Histone Post‐Translational Modifications and Histone Variants in Caenorhabditis elegans
por: Millan‐Ariño, Lluís, et al.
Publicado: (2020)

Identification of Novel Surface-Exposed Proteins of Rickettsia rickettsii by Affinity Purification and Proteomics
por: Gong, Wenping, et al.
Publicado: (2014)

A computational protein design protocol for optimization of the SARS-CoV-2 receptor-binding-motif affinity for human ACE2
por: Polydorides, Savvas, et al.
Publicado: (2022)

Convenient Protocol for Production and Purification of Clostridioides difficile Spores for Germination Studies
por: Weldy, Melissa, et al.
Publicado: (2020)

Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies
por: Li, Danqing, et al.
Publicado: (2022)

Protocol for the isolation and purification of human follicular dendritic cells for functional assays
por: Breeuwsma, Martijn, et al.
Publicado: (2023)

Protocol to measure bacterial intake and gut clearance of Caenorhabditis elegans
por: Wibisono, Phillip, et al.
Publicado: (2022)

An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease
por: Teng, Allen C.T., et al.
Publicado: (2022)

Protocol for purification of cells in their native state using reversible aptamer-antidote pairs
por: Requena, Martin D., et al.
Publicado: (2023)

Protocol for production and purification of SARS-CoV-2 3CL(pro)
por: Mazzei, Luca, et al.
Publicado: (2023)

Non-Affinity Purification of Antibodies
por: Arakawa, Tsutomu, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_p2icbvnjfd8ef4un5eo85q998f