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Direct fluorescent labeling of NF186 and Na(V)1.6 in living primary neurons using bioorthogonal click chemistry

The axon initial segment (AIS) is a highly specialized neuronal compartment that regulates the generation of action potentials and maintenance of neuronal polarity. Live imaging of the AIS is challenging due to the limited number of suitable labeling methods. To overcome this limitation, we establis...

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Detalles Bibliográficos
Autores principales: Stajković, Nevena, Liu, Yuanyuan, Arsić, Aleksandra, Meng, Ning, Lyu, Hang, Zhang, Nan, Grimm, Dirk, Lerche, Holger, Nikić-Spiegel, Ivana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323244/
https://www.ncbi.nlm.nih.gov/pubmed/37288813
http://dx.doi.org/10.1242/jcs.260600