Cargando…
Fast, precise and cloning-free knock-in of reporter sequences in vivo with high efficiency
Targeted knock-in of fluorescent reporters enables powerful gene and protein analyses in a physiological context. However, precise integration of long sequences remains challenging in vivo. Here, we demonstrate cloning-free and precise reporter knock-in into zebrafish genes, using PCR-generated temp...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Company of Biologists Ltd
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323249/ https://www.ncbi.nlm.nih.gov/pubmed/37309812 http://dx.doi.org/10.1242/dev.201323 |