SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries

Multiplexed assays of variant effects (MAVEs) have made possible the functional assessment of all possible mutations to genes and regulatory sequences. A core pillar of the approach is generation of variant libraries, but current methods are either difficult to scale or not uniform enough to enable...

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Detalles Bibliográficos
Autores principales: Mighell, Taylor L., Toledano, Ignasi, Lehner, Ben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328370/
https://www.ncbi.nlm.nih.gov/pubmed/37418460
http://dx.doi.org/10.1371/journal.pone.0288158

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328370/
https://www.ncbi.nlm.nih.gov/pubmed/37418460
http://dx.doi.org/10.1371/journal.pone.0288158

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