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Multiplex enzymatic synthesis of DNA with single-base resolution

Enzymatic DNA synthesis (EDS) is a promising benchtop and user-friendly method of nucleic acid synthesis that, instead of solvents and phosphoramidites, uses mild aqueous conditions and enzymes. For applications such as protein engineering and spatial transcriptomics that require either oligo pools...

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Detalles Bibliográficos
Autores principales: Verardo, Damiano, Adelizzi, Beatrice, Rodriguez-Pinzon, Daniel A., Moghaddam, Nicolas, Thomée, Emma, Loman, Tessa, Godron, Xavier, Horgan, Adrian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328407/
https://www.ncbi.nlm.nih.gov/pubmed/37418522
http://dx.doi.org/10.1126/sciadv.adi0263