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Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics

Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell transcrip...

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Detalles Bibliográficos
Autores principales: Zhang, Junyi, Straehle, Jakob, Joseph, Kevin, Neidert, Nicolas, Behringer, Simon, Göldner, Jonathan, Vlachos, Andreas, Prinz, Marco, Fung, Christian, Beck, Jürgen, Schnell, Oliver, Heiland, Dieter Henrik, Ravi, Vidhya M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328984/
https://www.ncbi.nlm.nih.gov/pubmed/37393609
http://dx.doi.org/10.1016/j.xpro.2023.102383