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Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system

In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live cell imaging system. We describe steps for culture of f...

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Detalles Bibliográficos
Autores principales: Ladaigue, Ségolène, Paget, Vincent, Lefranc, Anne-Charlotte, Quitoco, Monica, Bacquer, Emilie, Milliat, Fabien, Guipaud, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331580/
https://www.ncbi.nlm.nih.gov/pubmed/37379221
http://dx.doi.org/10.1016/j.xpro.2023.102388