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Protocol for the isolation and purification of human follicular dendritic cells for functional assays

In this protocol, we detail how to isolate and purify human follicular dendritic cells (FDCs) from lymphoid tissues. FDCs play a vital role in antibody development by presenting antigens to B cells in germinal centers. The assay involves enzymatic digestion and fluorescence-activated cell sorting an...

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Detalles Bibliográficos
Autores principales: Breeuwsma, Martijn, Heesters, Balthasar A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336301/
https://www.ncbi.nlm.nih.gov/pubmed/37392392
http://dx.doi.org/10.1016/j.xpro.2023.102404