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Protocol for the isolation and purification of human follicular dendritic cells for functional assays
In this protocol, we detail how to isolate and purify human follicular dendritic cells (FDCs) from lymphoid tissues. FDCs play a vital role in antibody development by presenting antigens to B cells in germinal centers. The assay involves enzymatic digestion and fluorescence-activated cell sorting an...
Autores principales: | Breeuwsma, Martijn, Heesters, Balthasar A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336301/ https://www.ncbi.nlm.nih.gov/pubmed/37392392 http://dx.doi.org/10.1016/j.xpro.2023.102404 |
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