Quantifying mitochondrial redox and bilirubin content in intact primary hepatocytes of obese mice using fluorescent reporters

Assessing the physiological role of H(2)O(2) requires sensitive techniques to quantify H(2)O(2) and antioxidants in live cells. Here, we present a protocol to assess the mitochondrial redox state and unconjugated bilirubin levels in intact live primary hepatocytes from obese mice. We described steps...

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Detalles Bibliográficos
Autores principales: Belmas, Thomas, Liesa, Marc, Shum, Michaël
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336327/
https://www.ncbi.nlm.nih.gov/pubmed/37393613
http://dx.doi.org/10.1016/j.xpro.2023.102408

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336327/
https://www.ncbi.nlm.nih.gov/pubmed/37393613
http://dx.doi.org/10.1016/j.xpro.2023.102408

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