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Quantifying mitochondrial redox and bilirubin content in intact primary hepatocytes of obese mice using fluorescent reporters
Assessing the physiological role of H(2)O(2) requires sensitive techniques to quantify H(2)O(2) and antioxidants in live cells. Here, we present a protocol to assess the mitochondrial redox state and unconjugated bilirubin levels in intact live primary hepatocytes from obese mice. We described steps...
Autores principales: | Belmas, Thomas, Liesa, Marc, Shum, Michaël |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336327/ https://www.ncbi.nlm.nih.gov/pubmed/37393613 http://dx.doi.org/10.1016/j.xpro.2023.102408 |
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