Cargando…

An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate

Generation of functionally mature organs requires exquisite control of transcriptional programs governing cell state transitions during development. Despite advances in understanding the behavior of adult intestinal stem cells and their progeny, the transcriptional regulators that control the emerge...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hansen, Stine L., Larsen, Hjalte L., Pikkupeura, Laura M., Maciag, Grzegorz, Guiu, Jordi, Müller, Iris, Clement, Ditte L., Mueller, Christina, Johansen, Jens Vilstrup, Helin, Kristian, Lerdrup, Mads, Jensen, Kim B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Association for the Advancement of Science 2023
Materias:	Biomedicine and Life Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10337909/ https://www.ncbi.nlm.nih.gov/pubmed/37436979 http://dx.doi.org/10.1126/sciadv.adg4055

Ejemplares similares

Transcriptional and epigenomic profiling identifies YAP signaling as a key regulator of intestinal epithelium maturation
por: Pikkupeura, Laura M., et al.
Publicado: (2023)

Organoid cell fate dynamics in space and time
por: Zheng, Xuan, et al.
Publicado: (2023)

Targeted gene silencing in the nervous system with CRISPR-Cas13
por: Powell, Jackson E., et al.
Publicado: (2022)

Analysis of bacterial pangenomes reduces CRISPR dark matter and reveals strong association between membranome and CRISPR-Cas systems
por: Rubio, Alejandro, et al.
Publicado: (2023)

Personalized chordoma organoids for drug discovery studies
por: Al Shihabi, Ahmad, et al.
Publicado: (2022)

Early SRC activation skews cell fate from apoptosis to senescence
por: Anerillas, Carlos, et al.
Publicado: (2022)

A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing
por: Wang, Yao, et al.
Publicado: (2023)

Transient agonism of the sonic hedgehog pathway triggers a permanent transition of skin appendage fate in the chicken embryo
por: Cooper, Rory L., et al.
Publicado: (2023)

Genome-editing prodrug: Targeted delivery and conditional stabilization of CRISPR-Cas9 for precision therapy of inflammatory disease
por: Yan, Xiaojie, et al.
Publicado: (2021)

Blood-brain barrier–penetrating single CRISPR-Cas9 nanocapsules for effective and safe glioblastoma gene therapy
por: Zou, Yan, et al.
Publicado: (2022)

Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editing
por: Park, So Hyun, et al.
Publicado: (2022)

Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system
por: Shiriaeva, Anna A., et al.
Publicado: (2022)

Efficient cancer modeling through CRISPR-Cas9/HDR-based somatic precision gene editing in mice
por: Bu, Wen, et al.
Publicado: (2023)

Transplanted organoids empower human preclinical assessment of drug candidate for the clinic
por: Westerling-Bui, Amy D., et al.
Publicado: (2022)

A BMP-2–triggered in vivo osteo-organoid for cell therapy
por: Dai, Kai, et al.
Publicado: (2023)

TAp63 determines the fate of oocytes against DNA damage
por: Luan, Yi, et al.
Publicado: (2022)

A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation
por: Wang, Xinyi, et al.
Publicado: (2021)

Precise CRISPR-Cas–mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells
por: Tran, Ngoc Tung, et al.
Publicado: (2022)

CRISPR-based engineering of RNA viruses
por: Nemudryi, Artem, et al.
Publicado: (2023)

In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapy
por: Han, Jeong Pil, et al.
Publicado: (2022)

Hierarchical self-uncloaking CRISPR-Cas13a–customized RNA nanococoons for spatial-controlled genome editing and precise cancer therapy
por: Fan, Ningke, et al.
Publicado: (2022)

Discoidin domain receptor 2 regulates aberrant mesenchymal lineage cell fate and matrix organization
por: Pagani, Chase A., et al.
Publicado: (2022)

Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
por: Hiratsuka, Ken, et al.
Publicado: (2022)

CRISPR-Cas9 screen reveals a role of purine synthesis for estrogen receptor α activity and tamoxifen resistance of breast cancer cells
por: Hany, Dina, et al.
Publicado: (2023)

In situ modulation of intestinal organoid epithelial curvature through photoinduced viscoelasticity directs crypt morphogenesis
por: Yavitt, F. Max, et al.
Publicado: (2023)

Nanowired human cardiac organoid transplantation enables highly efficient and effective recovery of infarcted hearts
por: Tan, Yu, et al.
Publicado: (2023)

A human mucosal melanoma organoid platform for modeling tumor heterogeneity and exploring immunotherapy combination options
por: Sun, Lulu, et al.
Publicado: (2023)

Recapitulating hepatitis E virus–host interactions and facilitating antiviral drug discovery in human liver–derived organoids
por: Li, Pengfei, et al.
Publicado: (2022)

Elongation factor-2 kinase is a critical determinant of the fate and antitumor immunity of CD8(+) T cells
por: Das, Jugal Kishore, et al.
Publicado: (2022)

GATA3 mediates nonclassical β-catenin signaling in skeletal cell fate determination and ectopic chondrogenesis
por: Maruyama, Takamitsu, et al.
Publicado: (2022)

GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate
por: Song, Mengyi, et al.
Publicado: (2022)

Single-cell profiling of alveolar rhabdomyosarcoma reveals RAS pathway inhibitors as cell-fate hijackers with therapeutic relevance
por: Danielli, Sara G., et al.
Publicado: (2023)

WUSCHEL-RELATED HOMEOBOX 13 suppresses de novo shoot regeneration via cell fate control of pluripotent callus
por: Ogura, Nao, et al.
Publicado: (2023)

A universal bacteriophage T4 nanoparticle platform to design multiplex SARS-CoV-2 vaccine candidates by CRISPR engineering
por: Zhu, Jingen, et al.
Publicado: (2021)

Optogenetic modeling of human neuromuscular circuits in Duchenne muscular dystrophy with CRISPR and pharmacological corrections
por: Paredes-Redondo, Amaia, et al.
Publicado: (2021)

Application of combined CRISPR screening for genetic and chemical-genetic interaction profiling in Mycobacterium tuberculosis
por: Yan, Mei-Yi, et al.
Publicado: (2022)

Endothelial versus pronephron fate decision is modulated by the transcription factors Cloche/Npas4l, Tal1, and Lmo2
por: Mattonet, Kenny, et al.
Publicado: (2022)

MKL/SRF and Bcl6 mutual transcriptional repression safeguards the fate and positioning of neocortical progenitor cells mediated by RhoA
por: Cossard, Alexia, et al.
Publicado: (2023)

Targeted DamID in C. elegans reveals a direct role for LIN-22 and NHR-25 in antagonizing the epidermal stem cell fate
por: Katsanos, Dimitris, et al.
Publicado: (2022)

Genome-wide CRISPR screens using isogenic cells reveal vulnerabilities conferred by loss of tumor suppressors
por: Feng, Xu, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_thc0i2gafojul3mgl6kqntvqdo