Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis

Bone regeneration is a complex process that requires the coordination of various biological events. Developing a tissue regeneration membrane that can regulate this cascade of events is challenging. In this study, we aimed to fabricate a membrane that can enrich the damaged area with mesenchymal ste...

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Autores principales: Al-Baadani, Mohammed A., Xu, Lihua, Cai, Kexin, Yie, Kendrick Hii Ru, Shen, Yiding, Al-Bishari, Abdullrahman M., Al-Shaaobi, Bilal A., Ma, Pingping, Shen, Xinkun, Liu, Jinsong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338360/
https://www.ncbi.nlm.nih.gov/pubmed/37455818
http://dx.doi.org/10.1016/j.mtbio.2023.100692
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author Al-Baadani, Mohammed A.
Xu, Lihua
Cai, Kexin
Yie, Kendrick Hii Ru
Shen, Yiding
Al-Bishari, Abdullrahman M.
Al-Shaaobi, Bilal A.
Ma, Pingping
Shen, Xinkun
Liu, Jinsong
author_facet Al-Baadani, Mohammed A.
Xu, Lihua
Cai, Kexin
Yie, Kendrick Hii Ru
Shen, Yiding
Al-Bishari, Abdullrahman M.
Al-Shaaobi, Bilal A.
Ma, Pingping
Shen, Xinkun
Liu, Jinsong
author_sort Al-Baadani, Mohammed A.
collection PubMed
description Bone regeneration is a complex process that requires the coordination of various biological events. Developing a tissue regeneration membrane that can regulate this cascade of events is challenging. In this study, we aimed to fabricate a membrane that can enrich the damaged area with mesenchymal stem cells, improve angiogenesis, and continuously induce osteogenesis. Our approach involved creating a hierarchical polycaprolactone/gelatin (PCL/GEL) co-electrospinning membrane that incorporated substance P (SP)-loaded GEL fibers and simvastatin (SIM)-loaded PCL fibers. The membrane could initiate a burst release of SP and a slow/sustained release of SIM for over a month. In vitro experiments, including those related to angiogenesis and osteogenesis (e.g., migration, endothelial network formation, alkaline phosphatase activity, mineralization, and gene expression), clearly demonstrated the membrane's superior ability to improve cell homing, revascularization, and osteogenic differentiation. Furthermore, a series of in vivo studies, including immunofluorescence of CD29(+)/CD90(+) double-positive cells and immunohistochemical staining for CD34 and vWF, confirmed the co-electrospinning membrane's ability to enhance MSC migration and revascularization response after five days of implantation. After one month, the Micro-CT and histological (Masson and H&E) results showed accelerated bone regeneration. Our findings suggest that a co-electrospinning membrane with time-tunable drug delivery could advance the development of tissue engineering therapeutic strategies and potentially improve patient outcomes.
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spelling pubmed-103383602023-07-14 Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis Al-Baadani, Mohammed A. Xu, Lihua Cai, Kexin Yie, Kendrick Hii Ru Shen, Yiding Al-Bishari, Abdullrahman M. Al-Shaaobi, Bilal A. Ma, Pingping Shen, Xinkun Liu, Jinsong Mater Today Bio Full Length Article Bone regeneration is a complex process that requires the coordination of various biological events. Developing a tissue regeneration membrane that can regulate this cascade of events is challenging. In this study, we aimed to fabricate a membrane that can enrich the damaged area with mesenchymal stem cells, improve angiogenesis, and continuously induce osteogenesis. Our approach involved creating a hierarchical polycaprolactone/gelatin (PCL/GEL) co-electrospinning membrane that incorporated substance P (SP)-loaded GEL fibers and simvastatin (SIM)-loaded PCL fibers. The membrane could initiate a burst release of SP and a slow/sustained release of SIM for over a month. In vitro experiments, including those related to angiogenesis and osteogenesis (e.g., migration, endothelial network formation, alkaline phosphatase activity, mineralization, and gene expression), clearly demonstrated the membrane's superior ability to improve cell homing, revascularization, and osteogenic differentiation. Furthermore, a series of in vivo studies, including immunofluorescence of CD29(+)/CD90(+) double-positive cells and immunohistochemical staining for CD34 and vWF, confirmed the co-electrospinning membrane's ability to enhance MSC migration and revascularization response after five days of implantation. After one month, the Micro-CT and histological (Masson and H&E) results showed accelerated bone regeneration. Our findings suggest that a co-electrospinning membrane with time-tunable drug delivery could advance the development of tissue engineering therapeutic strategies and potentially improve patient outcomes. Elsevier 2023-06-25 /pmc/articles/PMC10338360/ /pubmed/37455818 http://dx.doi.org/10.1016/j.mtbio.2023.100692 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Al-Baadani, Mohammed A.
Xu, Lihua
Cai, Kexin
Yie, Kendrick Hii Ru
Shen, Yiding
Al-Bishari, Abdullrahman M.
Al-Shaaobi, Bilal A.
Ma, Pingping
Shen, Xinkun
Liu, Jinsong
Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis
title Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis
title_full Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis
title_fullStr Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis
title_full_unstemmed Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis
title_short Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis
title_sort preparation of co-electrospinning membrane loaded with simvastatin and substance p to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338360/
https://www.ncbi.nlm.nih.gov/pubmed/37455818
http://dx.doi.org/10.1016/j.mtbio.2023.100692
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