Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis
Bone regeneration is a complex process that requires the coordination of various biological events. Developing a tissue regeneration membrane that can regulate this cascade of events is challenging. In this study, we aimed to fabricate a membrane that can enrich the damaged area with mesenchymal ste...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338360/ https://www.ncbi.nlm.nih.gov/pubmed/37455818 http://dx.doi.org/10.1016/j.mtbio.2023.100692 |
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author | Al-Baadani, Mohammed A. Xu, Lihua Cai, Kexin Yie, Kendrick Hii Ru Shen, Yiding Al-Bishari, Abdullrahman M. Al-Shaaobi, Bilal A. Ma, Pingping Shen, Xinkun Liu, Jinsong |
author_facet | Al-Baadani, Mohammed A. Xu, Lihua Cai, Kexin Yie, Kendrick Hii Ru Shen, Yiding Al-Bishari, Abdullrahman M. Al-Shaaobi, Bilal A. Ma, Pingping Shen, Xinkun Liu, Jinsong |
author_sort | Al-Baadani, Mohammed A. |
collection | PubMed |
description | Bone regeneration is a complex process that requires the coordination of various biological events. Developing a tissue regeneration membrane that can regulate this cascade of events is challenging. In this study, we aimed to fabricate a membrane that can enrich the damaged area with mesenchymal stem cells, improve angiogenesis, and continuously induce osteogenesis. Our approach involved creating a hierarchical polycaprolactone/gelatin (PCL/GEL) co-electrospinning membrane that incorporated substance P (SP)-loaded GEL fibers and simvastatin (SIM)-loaded PCL fibers. The membrane could initiate a burst release of SP and a slow/sustained release of SIM for over a month. In vitro experiments, including those related to angiogenesis and osteogenesis (e.g., migration, endothelial network formation, alkaline phosphatase activity, mineralization, and gene expression), clearly demonstrated the membrane's superior ability to improve cell homing, revascularization, and osteogenic differentiation. Furthermore, a series of in vivo studies, including immunofluorescence of CD29(+)/CD90(+) double-positive cells and immunohistochemical staining for CD34 and vWF, confirmed the co-electrospinning membrane's ability to enhance MSC migration and revascularization response after five days of implantation. After one month, the Micro-CT and histological (Masson and H&E) results showed accelerated bone regeneration. Our findings suggest that a co-electrospinning membrane with time-tunable drug delivery could advance the development of tissue engineering therapeutic strategies and potentially improve patient outcomes. |
format | Online Article Text |
id | pubmed-10338360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103383602023-07-14 Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis Al-Baadani, Mohammed A. Xu, Lihua Cai, Kexin Yie, Kendrick Hii Ru Shen, Yiding Al-Bishari, Abdullrahman M. Al-Shaaobi, Bilal A. Ma, Pingping Shen, Xinkun Liu, Jinsong Mater Today Bio Full Length Article Bone regeneration is a complex process that requires the coordination of various biological events. Developing a tissue regeneration membrane that can regulate this cascade of events is challenging. In this study, we aimed to fabricate a membrane that can enrich the damaged area with mesenchymal stem cells, improve angiogenesis, and continuously induce osteogenesis. Our approach involved creating a hierarchical polycaprolactone/gelatin (PCL/GEL) co-electrospinning membrane that incorporated substance P (SP)-loaded GEL fibers and simvastatin (SIM)-loaded PCL fibers. The membrane could initiate a burst release of SP and a slow/sustained release of SIM for over a month. In vitro experiments, including those related to angiogenesis and osteogenesis (e.g., migration, endothelial network formation, alkaline phosphatase activity, mineralization, and gene expression), clearly demonstrated the membrane's superior ability to improve cell homing, revascularization, and osteogenic differentiation. Furthermore, a series of in vivo studies, including immunofluorescence of CD29(+)/CD90(+) double-positive cells and immunohistochemical staining for CD34 and vWF, confirmed the co-electrospinning membrane's ability to enhance MSC migration and revascularization response after five days of implantation. After one month, the Micro-CT and histological (Masson and H&E) results showed accelerated bone regeneration. Our findings suggest that a co-electrospinning membrane with time-tunable drug delivery could advance the development of tissue engineering therapeutic strategies and potentially improve patient outcomes. Elsevier 2023-06-25 /pmc/articles/PMC10338360/ /pubmed/37455818 http://dx.doi.org/10.1016/j.mtbio.2023.100692 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Full Length Article Al-Baadani, Mohammed A. Xu, Lihua Cai, Kexin Yie, Kendrick Hii Ru Shen, Yiding Al-Bishari, Abdullrahman M. Al-Shaaobi, Bilal A. Ma, Pingping Shen, Xinkun Liu, Jinsong Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis |
title | Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis |
title_full | Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis |
title_fullStr | Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis |
title_full_unstemmed | Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis |
title_short | Preparation of co-electrospinning membrane loaded with simvastatin and substance P to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis |
title_sort | preparation of co-electrospinning membrane loaded with simvastatin and substance p to accelerate bone regeneration by promoting cell homing, angiogenesis and osteogenesis |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338360/ https://www.ncbi.nlm.nih.gov/pubmed/37455818 http://dx.doi.org/10.1016/j.mtbio.2023.100692 |
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