mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine

The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA anti...

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Detalles Bibliográficos
Autores principales: Gao, Xiaoyu, Li, Yao, Nie, Guangjun, Zhao, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338712/
https://www.ncbi.nlm.nih.gov/pubmed/37456344
http://dx.doi.org/10.21769/BioProtoc.4774
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author Gao, Xiaoyu
Li, Yao
Nie, Guangjun
Zhao, Xiao
author_facet Gao, Xiaoyu
Li, Yao
Nie, Guangjun
Zhao, Xiao
author_sort Gao, Xiaoyu
collection PubMed
description The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA antigens through L7Ae-boxC/D binding and deliver them into HEK-293T and dendritic cells. This platform provides an mRNA delivery technology distinct from lipid nanoparticles (LNPs) for personalized mRNA tumor vaccination and with a Plug-and-Display strategy suitable for rapid preparation of the personalized mRNA tumor vaccine against varied tumor antigens. Key features OMVs are employed as an mRNA delivery platform through L7Ae-boxC/D binding.
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spelling pubmed-103387122023-07-14 mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine Gao, Xiaoyu Li, Yao Nie, Guangjun Zhao, Xiao Bio Protoc Methods Article The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA antigens through L7Ae-boxC/D binding and deliver them into HEK-293T and dendritic cells. This platform provides an mRNA delivery technology distinct from lipid nanoparticles (LNPs) for personalized mRNA tumor vaccination and with a Plug-and-Display strategy suitable for rapid preparation of the personalized mRNA tumor vaccine against varied tumor antigens. Key features OMVs are employed as an mRNA delivery platform through L7Ae-boxC/D binding. Bio-Protocol 2023-07-05 /pmc/articles/PMC10338712/ /pubmed/37456344 http://dx.doi.org/10.21769/BioProtoc.4774 Text en ©Copyright : © 2023 The Authors; This is an open access article under the CC BY license https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Methods Article
Gao, Xiaoyu
Li, Yao
Nie, Guangjun
Zhao, Xiao
mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
title mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
title_full mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
title_fullStr mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
title_full_unstemmed mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
title_short mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
title_sort mrna delivery platform based on bacterial outer membrane vesicles for tumor vaccine
topic Methods Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338712/
https://www.ncbi.nlm.nih.gov/pubmed/37456344
http://dx.doi.org/10.21769/BioProtoc.4774
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