Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometry

Single-cell RNA sequencing (scRNA-seq) requires the preparation of a highly viable single-cell suspension to get reliable sequencing results. Here, we present a protocol for isolating mouse footpad leukocytes while maintaining high viability. We describe steps for footpad collection, enzymatic tissu...

Descripción completa

Detalles Bibliográficos
Autores principales: Hameed, Muddassar, Rai, Pallavi, Makris, Melissa, Weger-Lucarelli, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339044/
https://www.ncbi.nlm.nih.gov/pubmed/37402171
http://dx.doi.org/10.1016/j.xpro.2023.102409

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339044/
https://www.ncbi.nlm.nih.gov/pubmed/37402171
http://dx.doi.org/10.1016/j.xpro.2023.102409

Ejemplares similares