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Antisera Produced Using an E. coli-Expressed SARS-CoV-2 RBD and Complemented with a Minimal Dose of Mammalian-Cell-Expressed S1 Subunit of the Spike Protein Exhibits Improved Neutralization
E. coli-expressed proteins could provide a rapid, cost-effective, and safe antigen for subunit vaccines, provided we can produce them in a properly folded form inducing neutralizing antibodies. Here, we use an E. coli-expressed SARS-CoV-2 receptor-binding domain (RBD) of the spike protein as a model...
Autores principales: | Yoshizue, Takahiro, Brindha, Subbaian, Wongnak, Rawiwan, Takemae, Hitoshi, Oba, Mami, Mizutani, Tetsuya, Kuroda, Yutaka |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341788/ https://www.ncbi.nlm.nih.gov/pubmed/37445760 http://dx.doi.org/10.3390/ijms241310583 |
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