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Comparison of phasor analysis and biexponential decay curve fitting of autofluorescence lifetime imaging data for machine learning prediction of cellular phenotypes

Introduction: Autofluorescence imaging of the coenzymes reduced nicotinamide (phosphate) dinucleotide (NAD(P)H) and oxidized flavin adenine dinucleotide (FAD) provides a label-free method to detect cellular metabolism and phenotypes. Time-domain fluorescence lifetime data can be analyzed by exponent...

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Detalles Bibliográficos
Autores principales: Hu, Linghao, Ter Hofstede, Blanche, Sharma, Dhavan, Zhao, Feng, Walsh, Alex J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342207/
https://www.ncbi.nlm.nih.gov/pubmed/37455808
http://dx.doi.org/10.3389/fbinf.2023.1210157