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Comparison of Four Methods of RNA Extraction and cDNA Synthesis from The Venom of Peruvian Snakes of the Genus Bothrops of Clinical Importance

RNA purification and cDNA synthesis represents the starting point for molecular analyses of snake venom proteins-enzymes. Usually, the sacrifice of snakes is necessary for venom gland extraction to identify protein-coding transcripts; however, the venom can be used as a source of transcripts. Althou...

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Detalles Bibliográficos
Autores principales: Torrejón, Daniel, Cárdenas, Javier, Juárez, Diana, Espinoza, Jordano, Proleón, Alex, Agurto-Arteaga, Andrés, Lazo, Fanny, Leguía, Mariana, Urra, Félix A., Sánchez, Eladio F., Chávez-Olortegui, Carlos, Vivas-Ruiz, Dan E., Yarlequé, Armando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342303/
https://www.ncbi.nlm.nih.gov/pubmed/37446341
http://dx.doi.org/10.3390/ijms241311161