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Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

Generation of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process of outcrossing to eliminate CRISPR–Cas9-associated sequences and produce transgene-free lines. We have addressed this is...

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Detalles Bibliográficos
Autores principales: Yang, Lei, Machin, Frank, Wang, Shuangfeng, Saplaoura, Eleftheria, Kragler, Friedrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10344777/
https://www.ncbi.nlm.nih.gov/pubmed/36593415
http://dx.doi.org/10.1038/s41587-022-01585-8