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Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
Generation of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process of outcrossing to eliminate CRISPR–Cas9-associated sequences and produce transgene-free lines. We have addressed this is...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10344777/ https://www.ncbi.nlm.nih.gov/pubmed/36593415 http://dx.doi.org/10.1038/s41587-022-01585-8 |