Glyoxal-based fixation of Drosophila embryos for immunofluorescence staining and RNA in situ hybridization

The dialdehyde glyoxal is an alternative chemical fixative that cross-links tissues faster than formaldehyde, retains higher antigenicity, and is less hazardous than either formaldehyde or glutaraldehyde. Here we present a glyoxal-based fixation protocol for use with Drosophila embryos. We describe...

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Detalles Bibliográficos
Autores principales: Amin, Shrunali, Basu, Malika, Buzinova, Valeria, Delgado, Anthony, Mahadevan, Tejas, Mishra, Sanya, Zaida, Sarah, Wang, Xi, Sokac, Anna Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345161/
https://www.ncbi.nlm.nih.gov/pubmed/37405926
http://dx.doi.org/10.1016/j.xpro.2023.102385

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345161/
https://www.ncbi.nlm.nih.gov/pubmed/37405926
http://dx.doi.org/10.1016/j.xpro.2023.102385

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