Detection of membrane-anchoring lipid modifications of proteins in cells by radioactive metabolic labeling

Prenylation and palmitoylation are two major lipid modifications of cellular proteins that anchor proteins to cell membranes. Here, we present a protocol for detecting these modifications in cellular proteins by radioactive metabolic labeling. We describe steps for metabolic labeling of cells, cell...

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Detalles Bibliográficos
Autores principales: Bhat, Sameer Ahmed, Ferguson, Rachel, Adhikari, Ritika, Kuchay, Shafi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345189/
https://www.ncbi.nlm.nih.gov/pubmed/37405928
http://dx.doi.org/10.1016/j.xpro.2023.102416

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345189/
https://www.ncbi.nlm.nih.gov/pubmed/37405928
http://dx.doi.org/10.1016/j.xpro.2023.102416

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