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Detection of membrane-anchoring lipid modifications of proteins in cells by radioactive metabolic labeling
Prenylation and palmitoylation are two major lipid modifications of cellular proteins that anchor proteins to cell membranes. Here, we present a protocol for detecting these modifications in cellular proteins by radioactive metabolic labeling. We describe steps for metabolic labeling of cells, cell...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345189/ https://www.ncbi.nlm.nih.gov/pubmed/37405928 http://dx.doi.org/10.1016/j.xpro.2023.102416 |