Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers

Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xiao, Lam, Kevin, Zhao, Huayi, Sakane, Sadatsugu, Kim, Hyun Young, Eguileor, Alvaro, Diggle, Karin, Wu, Shuai, Gontijo Weber, Raquel Carvalho, Soroosh, Pejman, Hosseini, Mojgan, Mekeel, Kristin, Brenner, David A., Kisseleva, Tatiana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345194/
https://www.ncbi.nlm.nih.gov/pubmed/37405925
http://dx.doi.org/10.1016/j.xpro.2023.102391

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345194/
https://www.ncbi.nlm.nih.gov/pubmed/37405925
http://dx.doi.org/10.1016/j.xpro.2023.102391

Ejemplares similares