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Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers
Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345194/ https://www.ncbi.nlm.nih.gov/pubmed/37405925 http://dx.doi.org/10.1016/j.xpro.2023.102391 |
| _version_ | 1785073032441626624 |
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| author | Liu, Xiao Lam, Kevin Zhao, Huayi Sakane, Sadatsugu Kim, Hyun Young Eguileor, Alvaro Diggle, Karin Wu, Shuai Gontijo Weber, Raquel Carvalho Soroosh, Pejman Hosseini, Mojgan Mekeel, Kristin Brenner, David A. Kisseleva, Tatiana |
| author_facet | Liu, Xiao Lam, Kevin Zhao, Huayi Sakane, Sadatsugu Kim, Hyun Young Eguileor, Alvaro Diggle, Karin Wu, Shuai Gontijo Weber, Raquel Carvalho Soroosh, Pejman Hosseini, Mojgan Mekeel, Kristin Brenner, David A. Kisseleva, Tatiana |
| author_sort | Liu, Xiao |
| collection | PubMed |
| description | Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research. |
| format | Online Article Text |
| id | pubmed-10345194 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-103451942023-07-15 Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers Liu, Xiao Lam, Kevin Zhao, Huayi Sakane, Sadatsugu Kim, Hyun Young Eguileor, Alvaro Diggle, Karin Wu, Shuai Gontijo Weber, Raquel Carvalho Soroosh, Pejman Hosseini, Mojgan Mekeel, Kristin Brenner, David A. Kisseleva, Tatiana STAR Protoc Protocol Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research. Elsevier 2023-07-04 /pmc/articles/PMC10345194/ /pubmed/37405925 http://dx.doi.org/10.1016/j.xpro.2023.102391 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Protocol Liu, Xiao Lam, Kevin Zhao, Huayi Sakane, Sadatsugu Kim, Hyun Young Eguileor, Alvaro Diggle, Karin Wu, Shuai Gontijo Weber, Raquel Carvalho Soroosh, Pejman Hosseini, Mojgan Mekeel, Kristin Brenner, David A. Kisseleva, Tatiana Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers |
| title | Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers |
| title_full | Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers |
| title_fullStr | Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers |
| title_full_unstemmed | Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers |
| title_short | Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers |
| title_sort | isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers |
| topic | Protocol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345194/ https://www.ncbi.nlm.nih.gov/pubmed/37405925 http://dx.doi.org/10.1016/j.xpro.2023.102391 |
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