Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts

Transwell co-culture with human AC16 cardiomyocyte-like cells modifies the response of primary human ventricular fibroblasts to TGF-β stimulation. Fibrotic response markers including collagen I (COL1A1) and ɑ-smooth muscle actin (ACTA2) are amplified in the presence of AC16 cells, whereas others inc...

Descripción completa

Detalles Bibliográficos
Autores principales: Hidalgo, Veronica, Kastury, Nikhitha, Durham, Cheyanne W., Currie, Jordan, Amaya, Milton, Lam, Maggie P. Y., Lau, Edward
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349648/
https://www.ncbi.nlm.nih.gov/pubmed/37456138
http://dx.doi.org/10.17912/micropub.biology.000864
_version_ 1785073961630957568
author Hidalgo, Veronica
Kastury, Nikhitha
Durham, Cheyanne W.
Currie, Jordan
Amaya, Milton
Lam, Maggie P. Y.
Lau, Edward
author_facet Hidalgo, Veronica
Kastury, Nikhitha
Durham, Cheyanne W.
Currie, Jordan
Amaya, Milton
Lam, Maggie P. Y.
Lau, Edward
author_sort Hidalgo, Veronica
collection PubMed
description Transwell co-culture with human AC16 cardiomyocyte-like cells modifies the response of primary human ventricular fibroblasts to TGF-β stimulation. Fibrotic response markers including collagen I (COL1A1) and ɑ-smooth muscle actin (ACTA2) are amplified in the presence of AC16 cells, whereas others including periostin (POSTN) and fibronectin (FN1) are suppressed. Similar modulation is observed when the ventricular fibroblasts are co-cultured with AC16 cells under baseline and induced senescence conditions. Given that the response to TGF-β stimulation is commonly measured to study fibrotic signaling and drug treatments in vitro, the results here suggest that the effect of cellular crosstalk should be more broadly considered.
format Online
Article
Text
id pubmed-10349648
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Caltech Library
record_format MEDLINE/PubMed
spelling pubmed-103496482023-07-16 Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts Hidalgo, Veronica Kastury, Nikhitha Durham, Cheyanne W. Currie, Jordan Amaya, Milton Lam, Maggie P. Y. Lau, Edward MicroPubl Biol New Finding Transwell co-culture with human AC16 cardiomyocyte-like cells modifies the response of primary human ventricular fibroblasts to TGF-β stimulation. Fibrotic response markers including collagen I (COL1A1) and ɑ-smooth muscle actin (ACTA2) are amplified in the presence of AC16 cells, whereas others including periostin (POSTN) and fibronectin (FN1) are suppressed. Similar modulation is observed when the ventricular fibroblasts are co-cultured with AC16 cells under baseline and induced senescence conditions. Given that the response to TGF-β stimulation is commonly measured to study fibrotic signaling and drug treatments in vitro, the results here suggest that the effect of cellular crosstalk should be more broadly considered. Caltech Library 2023-06-29 /pmc/articles/PMC10349648/ /pubmed/37456138 http://dx.doi.org/10.17912/micropub.biology.000864 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Hidalgo, Veronica
Kastury, Nikhitha
Durham, Cheyanne W.
Currie, Jordan
Amaya, Milton
Lam, Maggie P. Y.
Lau, Edward
Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts
title Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts
title_full Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts
title_fullStr Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts
title_full_unstemmed Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts
title_short Co-culture with normal and senescent AC16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts
title_sort co-culture with normal and senescent ac16 cardiomyocytes modulates fibrotic response in primary human ventricular fibroblasts
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349648/
https://www.ncbi.nlm.nih.gov/pubmed/37456138
http://dx.doi.org/10.17912/micropub.biology.000864
work_keys_str_mv AT hidalgoveronica coculturewithnormalandsenescentac16cardiomyocytesmodulatesfibroticresponseinprimaryhumanventricularfibroblasts
AT kasturynikhitha coculturewithnormalandsenescentac16cardiomyocytesmodulatesfibroticresponseinprimaryhumanventricularfibroblasts
AT durhamcheyannew coculturewithnormalandsenescentac16cardiomyocytesmodulatesfibroticresponseinprimaryhumanventricularfibroblasts
AT curriejordan coculturewithnormalandsenescentac16cardiomyocytesmodulatesfibroticresponseinprimaryhumanventricularfibroblasts
AT amayamilton coculturewithnormalandsenescentac16cardiomyocytesmodulatesfibroticresponseinprimaryhumanventricularfibroblasts
AT lammaggiepy coculturewithnormalandsenescentac16cardiomyocytesmodulatesfibroticresponseinprimaryhumanventricularfibroblasts
AT lauedward coculturewithnormalandsenescentac16cardiomyocytesmodulatesfibroticresponseinprimaryhumanventricularfibroblasts

Ejemplares similares