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Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA

The procedure illustrated in this paper represents a new method for transcriptome analysis by PCR (Polymerase Chain Reaction), which circumvents the need for elimination of potential DNA contamination. Compared to the existing methodologies, our method is more precise, simpler and more reproducible...

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Detalles Bibliográficos
Autores principales: Đermić, Damir, Ljubić, Sven, Matulić, Maja, Procino, Alfredo, Feliciello, Maria Chiara, Ugarković, Đurđica, Feliciello, Isidoro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349872/
https://www.ncbi.nlm.nih.gov/pubmed/37454173
http://dx.doi.org/10.1038/s41598-023-38383-4