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Transgene Expression and Transposition Efficiency of Two-Component Sleeping Beauty Transposon Vector Systems Utilizing Plasmid or mRNA Encoding the Transposase

The use of two-component transposon plasmid vector systems, namely, a transposase construct and a donor vector carrying the gene of interest (GOI) can accelerate the development of recombinant cell lines. However, the undesired stable transfection of the transposase construct and the sustained expre...

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Detalles Bibliográficos
Autores principales: Tschorn, Natalie, van Heuvel, Yasemin, Stitz, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352156/
https://www.ncbi.nlm.nih.gov/pubmed/36547824
http://dx.doi.org/10.1007/s12033-022-00642-6