Structure and assembly of the NOT10:11 module of the CCR4-NOT complex

NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 te...

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Autores principales: Levdansky, Yevgen, Raisch, Tobias, Deme, Justin C., Pekovic, Filip, Elmlund, Hans, Lea, Susan M., Valkov, Eugene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352241/
https://www.ncbi.nlm.nih.gov/pubmed/37460791
http://dx.doi.org/10.1038/s42003-023-05122-4
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author Levdansky, Yevgen
Raisch, Tobias
Deme, Justin C.
Pekovic, Filip
Elmlund, Hans
Lea, Susan M.
Valkov, Eugene
author_facet Levdansky, Yevgen
Raisch, Tobias
Deme, Justin C.
Pekovic, Filip
Elmlund, Hans
Lea, Susan M.
Valkov, Eugene
author_sort Levdansky, Yevgen
collection PubMed
description NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 ternary complexes to sub-3 Å resolution, revealing an evolutionarily conserved, flexible structure. Through biochemical dissection studies, which include the Drosophila orthologs, we show that the module assembly is hierarchical, with NOT11 binding to NOT10, which then organizes it for binding to NOT1. A short proline-rich motif in NOT11 stabilizes the entire module assembly.
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spelling pubmed-103522412023-07-19 Structure and assembly of the NOT10:11 module of the CCR4-NOT complex Levdansky, Yevgen Raisch, Tobias Deme, Justin C. Pekovic, Filip Elmlund, Hans Lea, Susan M. Valkov, Eugene Commun Biol Article NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 ternary complexes to sub-3 Å resolution, revealing an evolutionarily conserved, flexible structure. Through biochemical dissection studies, which include the Drosophila orthologs, we show that the module assembly is hierarchical, with NOT11 binding to NOT10, which then organizes it for binding to NOT1. A short proline-rich motif in NOT11 stabilizes the entire module assembly. Nature Publishing Group UK 2023-07-17 /pmc/articles/PMC10352241/ /pubmed/37460791 http://dx.doi.org/10.1038/s42003-023-05122-4 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Levdansky, Yevgen
Raisch, Tobias
Deme, Justin C.
Pekovic, Filip
Elmlund, Hans
Lea, Susan M.
Valkov, Eugene
Structure and assembly of the NOT10:11 module of the CCR4-NOT complex
title Structure and assembly of the NOT10:11 module of the CCR4-NOT complex
title_full Structure and assembly of the NOT10:11 module of the CCR4-NOT complex
title_fullStr Structure and assembly of the NOT10:11 module of the CCR4-NOT complex
title_full_unstemmed Structure and assembly of the NOT10:11 module of the CCR4-NOT complex
title_short Structure and assembly of the NOT10:11 module of the CCR4-NOT complex
title_sort structure and assembly of the not10:11 module of the ccr4-not complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352241/
https://www.ncbi.nlm.nih.gov/pubmed/37460791
http://dx.doi.org/10.1038/s42003-023-05122-4
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