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USP12 promotes antiviral responses by deubiquitinating and stabilizing IFI16

Deubiquitinating enzymes (DUBs) regulate antiviral immune response through targeting DNA sensor signaling pathway members. As one of the DNA sensors, interferon (IFN)-γ inducible protein 16 (IFI16) play a major role in response to virus infections through activating the canonical STING/TBK-1/IRF3 si...

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Autores principales: Fu, Yuling, Zhan, Xiaoxia, You, Xiaolong, Nie, Dingnai, Mai, Haiyan, Chen, Yitian, He, Shitong, Sheng, Junli, Zeng, Zhijie, Li, Hongwei, Li, Jinlong, Hu, Shengfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353808/
https://www.ncbi.nlm.nih.gov/pubmed/37410794
http://dx.doi.org/10.1371/journal.ppat.1011480
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author Fu, Yuling
Zhan, Xiaoxia
You, Xiaolong
Nie, Dingnai
Mai, Haiyan
Chen, Yitian
He, Shitong
Sheng, Junli
Zeng, Zhijie
Li, Hongwei
Li, Jinlong
Hu, Shengfeng
author_facet Fu, Yuling
Zhan, Xiaoxia
You, Xiaolong
Nie, Dingnai
Mai, Haiyan
Chen, Yitian
He, Shitong
Sheng, Junli
Zeng, Zhijie
Li, Hongwei
Li, Jinlong
Hu, Shengfeng
author_sort Fu, Yuling
collection PubMed
description Deubiquitinating enzymes (DUBs) regulate antiviral immune response through targeting DNA sensor signaling pathway members. As one of the DNA sensors, interferon (IFN)-γ inducible protein 16 (IFI16) play a major role in response to virus infections through activating the canonical STING/TBK-1/IRF3 signaling pathway. Only a few studies discuss the function of DUBs in IFI16-mediated antiviral response. Ubiquitin-specific protease 12 (USP12), which is one of the major members of the USP family, participates in various biological functions. However, whether USP12 regulates the nucleic acid sensor to modulate antiviral immune responses has not yet been elucidated. In this study, we found that knockout or knockdown of USP12 impaired the HSV-1-induced expressions of IFN-β, CCL-5, IL-6, and downstream interferon-stimulated genes (ISGs). Moreover, USP12 deficiency increased HSV-1 replication and host susceptibility to HSV-1 infection. Mechanistically, USP12 inhibited the proteasome-dependent degradation of IFI16 through its deubiquitinase activity, thereby maintaining IFI16 stability and promoting IFI16-STING-IRF3- and p65-mediated antiviral signaling. Overall, our findings demonstrate an essential role of USP12 in DNA-sensing signaling and contribute to the understanding of deubiquitination-mediated regulation of innate antiviral responses.
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spelling pubmed-103538082023-07-19 USP12 promotes antiviral responses by deubiquitinating and stabilizing IFI16 Fu, Yuling Zhan, Xiaoxia You, Xiaolong Nie, Dingnai Mai, Haiyan Chen, Yitian He, Shitong Sheng, Junli Zeng, Zhijie Li, Hongwei Li, Jinlong Hu, Shengfeng PLoS Pathog Research Article Deubiquitinating enzymes (DUBs) regulate antiviral immune response through targeting DNA sensor signaling pathway members. As one of the DNA sensors, interferon (IFN)-γ inducible protein 16 (IFI16) play a major role in response to virus infections through activating the canonical STING/TBK-1/IRF3 signaling pathway. Only a few studies discuss the function of DUBs in IFI16-mediated antiviral response. Ubiquitin-specific protease 12 (USP12), which is one of the major members of the USP family, participates in various biological functions. However, whether USP12 regulates the nucleic acid sensor to modulate antiviral immune responses has not yet been elucidated. In this study, we found that knockout or knockdown of USP12 impaired the HSV-1-induced expressions of IFN-β, CCL-5, IL-6, and downstream interferon-stimulated genes (ISGs). Moreover, USP12 deficiency increased HSV-1 replication and host susceptibility to HSV-1 infection. Mechanistically, USP12 inhibited the proteasome-dependent degradation of IFI16 through its deubiquitinase activity, thereby maintaining IFI16 stability and promoting IFI16-STING-IRF3- and p65-mediated antiviral signaling. Overall, our findings demonstrate an essential role of USP12 in DNA-sensing signaling and contribute to the understanding of deubiquitination-mediated regulation of innate antiviral responses. Public Library of Science 2023-07-06 /pmc/articles/PMC10353808/ /pubmed/37410794 http://dx.doi.org/10.1371/journal.ppat.1011480 Text en © 2023 Fu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fu, Yuling
Zhan, Xiaoxia
You, Xiaolong
Nie, Dingnai
Mai, Haiyan
Chen, Yitian
He, Shitong
Sheng, Junli
Zeng, Zhijie
Li, Hongwei
Li, Jinlong
Hu, Shengfeng
USP12 promotes antiviral responses by deubiquitinating and stabilizing IFI16
title USP12 promotes antiviral responses by deubiquitinating and stabilizing IFI16
title_full USP12 promotes antiviral responses by deubiquitinating and stabilizing IFI16
title_fullStr USP12 promotes antiviral responses by deubiquitinating and stabilizing IFI16
title_full_unstemmed USP12 promotes antiviral responses by deubiquitinating and stabilizing IFI16
title_short USP12 promotes antiviral responses by deubiquitinating and stabilizing IFI16
title_sort usp12 promotes antiviral responses by deubiquitinating and stabilizing ifi16
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353808/
https://www.ncbi.nlm.nih.gov/pubmed/37410794
http://dx.doi.org/10.1371/journal.ppat.1011480
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