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Assessment of enzymatically synthesized DNA for gene assembly

Phosphoramidite chemical DNA synthesis technology is utilized for creating de novo ssDNA building blocks and is widely used by commercial vendors. Recent advances in enzymatic DNA synthesis (EDS), including engineered enzymes and reversibly terminated nucleotides, bring EDS technology into competiti...

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Detalles Bibliográficos
Autores principales: Simmons, Brooke L., McDonald, Nathan D., Robinett, Natalie G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354541/
https://www.ncbi.nlm.nih.gov/pubmed/37476479
http://dx.doi.org/10.3389/fbioe.2023.1208784