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Strategies to Improve Multi-enzyme Compatibility and Coordination in One-Pot SHERLOCK

[Image: see text] While molecular diagnostics generally require heating elements that supply high temperatures such as 95 °C in polymerase chain reaction and 60–69 °C in loop-mediated isothermal amplification, the recently developed CRISPR-based SHERLOCK (specific high-sensitivity enzymatic reporter...

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Detalles Bibliográficos
Autores principales: Li, Hongzhao, Kielich, Dominic M. S., Liu, Guodong, Smith, Greg, Bello, Alexander, Strong, James E., Pickering, Bradley S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357406/
https://www.ncbi.nlm.nih.gov/pubmed/37390127
http://dx.doi.org/10.1021/acs.analchem.2c05032