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Development of bright red‐shifted miRFP704nano using structural analysis of miRFPnano proteins

We recently converted the GAF domain of NpR3784 cyanobacteriochrome into near‐infrared (NIR) fluorescent proteins (FPs). Unlike cyanobacterichrome, which incorporates phycocyanobilin tetrapyrrole, engineered NIR FPs bind biliverdin abundant in mammalian cells, thus being the smallest scaffold for it...

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Detalles Bibliográficos
Autores principales: Oliinyk, Olena S., Pletnev, Sergei, Baloban, Mikhail, Verkhusha, Vladislav V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357936/
https://www.ncbi.nlm.nih.gov/pubmed/37347539
http://dx.doi.org/10.1002/pro.4709