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CREEPY: CRISPR-mediated editing of synthetic episomes in yeast

Use of synthetic genomics to design and build ‘big’ DNA has revolutionized our ability to answer fundamental biological questions by employing a bottom-up approach. Saccharomyces cerevisiae, or budding yeast, has become the major platform to assemble large synthetic constructs thanks to its powerful...

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Detalles Bibliográficos
Autores principales: Zhao, Yu, Coelho, Camila, Lauer, Stephanie, Majewski, Miłosz, Laurent, Jon M, Brosh, Ran, Boeke, Jef D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10359617/
https://www.ncbi.nlm.nih.gov/pubmed/37326023
http://dx.doi.org/10.1093/nar/gkad491