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Comparison of NET quantification methods based on immunofluorescence microscopy: Hand-counting, semi-automated and automated evaluations

Formation of neutrophil extracellular traps was first described in 2004, showing that NETs are composed of decondensed chromatin fibers and nuclear and granule components. Free DNA is often used to quantify NETs, but to differentiate NETosis from necrotic DNA-release, immunofluorescence microscopy w...

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Detalles Bibliográficos
Autores principales: Henneck, Timo, Krüger, Christina, Nerlich, Andreas, Langer, Melissa, Fingerhut, Leonie, Bonilla, Marta C., Meurer, Marita, von den Berg, Sönke, de Buhr, Nicole, Branitzki-Heinemann, Katja, von Köckritz-Blickwede, Maren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10361044/
https://www.ncbi.nlm.nih.gov/pubmed/37484269
http://dx.doi.org/10.1016/j.heliyon.2023.e16982