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Optimization of in vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation of soybean for visual screening of transformants using RUBY

In vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation (HRT) assays are key components of the plant biotechnology and functional genomics toolkit. In this report, both in vitro and ex vitro HRT were optimized in soybean using the RUBY reporter. Different parameters includi...

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Detalles Bibliográficos
Autores principales: Niazian, Mohsen, Belzile, François, Curtin, Shaun J., de Ronne, Maxime, Torkamaneh, Davoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10361064/
https://www.ncbi.nlm.nih.gov/pubmed/37484469
http://dx.doi.org/10.3389/fpls.2023.1207762