Serum-free lentiviral vector production is compatible with medium-resident nuclease activity arising from adherent HEK293T host cells engineered with a nuclease-encoding transgene

At present lentiviral vector production for cell and gene therapy commonly involves transient plasmid transfection of mammalian cells cultivated in serum-containing media and addition of exogenous nuclease to reduce host cell and plasmid DNA impurities. Switching from serum-containing media to chemi...

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Detalles Bibliográficos
Autores principales: Ali, Sadfer, Rivera, Milena, Ward, John, Keshavarz-Moore, Eli, Mason, Chris, Nesbeth, Darren N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10361239/
https://www.ncbi.nlm.nih.gov/pubmed/37484388
http://dx.doi.org/10.1016/j.heliyon.2023.e17067

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10361239/
https://www.ncbi.nlm.nih.gov/pubmed/37484388
http://dx.doi.org/10.1016/j.heliyon.2023.e17067

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