Generation of gene-of-interest double allele knockout clones in primary human T cells by CRISPR

Gene-of-interest (GOI) knockout is an important technique to study the genetic mechanisms of T cells. Here, we present a protocol to generate GOI double allele gene knockouts in primary human T cells by CRISPR, thus depleting proteins of interest expressed intracellularly or extracellularly in prima...

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Detalles Bibliográficos
Autores principales: Wu, Ling, Tan, Jia Chi, Gascoigne, Nicholas R.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362176/
https://www.ncbi.nlm.nih.gov/pubmed/37432856
http://dx.doi.org/10.1016/j.xpro.2023.102445

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362176/
https://www.ncbi.nlm.nih.gov/pubmed/37432856
http://dx.doi.org/10.1016/j.xpro.2023.102445

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