Mapping alternative polyadenylation in human cells using direct RNA sequencing technology

Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate mRNA isoforms with alternative 3′ untranslated regions. Here, we detail a protocol for detecting APA genome wide using direct RNA sequencing technology including computational analysis. We describe steps for RNA sam...

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Detalles Bibliográficos
Autores principales: Polenkowski, Mareike, Allister, Aldrige Bernardus, Burbano de Lara, Sebastian, Soltau, Madleen, Kendre, Gajanan, Tran, Doan Duy Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362186/
https://www.ncbi.nlm.nih.gov/pubmed/37432858
http://dx.doi.org/10.1016/j.xpro.2023.102420

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362186/
https://www.ncbi.nlm.nih.gov/pubmed/37432858
http://dx.doi.org/10.1016/j.xpro.2023.102420

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