Cargando…

Mapping alternative polyadenylation in human cells using direct RNA sequencing technology

Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate mRNA isoforms with alternative 3′ untranslated regions. Here, we detail a protocol for detecting APA genome wide using direct RNA sequencing technology including computational analysis. We describe steps for RNA sam...

Descripción completa

Detalles Bibliográficos
Autores principales:	Polenkowski, Mareike, Allister, Aldrige Bernardus, Burbano de Lara, Sebastian, Soltau, Madleen, Kendre, Gajanan, Tran, Doan Duy Hai
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362186/ https://www.ncbi.nlm.nih.gov/pubmed/37432858 http://dx.doi.org/10.1016/j.xpro.2023.102420

Ejemplares similares

C20orf204, a hepatocellular carcinoma-specific protein interacts with nucleolin and promotes cell proliferation
por: Burbano De Lara, Sebastian, et al.
Publicado: (2021)

Identification of Novel Micropeptides Derived from Hepatocellular Carcinoma-Specific Long Noncoding RNA
por: Polenkowski, Mareike, et al.
Publicado: (2021)

THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate
por: Polenkowski, Mareike, et al.
Publicado: (2022)

Using population-scale transcriptomic and genomic data to map 3′ UTR alternative polyadenylation quantitative trait loci
por: Zou, Xudong, et al.
Publicado: (2022)

Myc/Max dependent intronic long antisense noncoding RNA, EVA1A-AS, suppresses the expression of Myc/Max dependent anti-proliferating gene EVA1A in a U2 dependent manner
por: Niehus, Svenja E., et al.
Publicado: (2019)

Genome-wide mapping of polyadenylation sites in fission yeast reveals widespread alternative polyadenylation
por: Mata, Juan
Publicado: (2013)

Alternative polyadenylation of cyclooxygenase-2
por: Hall-Pogar, Tyra, et al.
Publicado: (2005)

Alternative Polyadenylation in Human Diseases
por: Chang, Jae-Woong, et al.
Publicado: (2017)

Analyses of alternative polyadenylation: from old school biochemistry to high-throughput technologies
por: Yeh, Hsin-Sung, et al.
Publicado: (2017)

Biased alternative polyadenylation in human tissues
por: Zhang, Haibo, et al.
Publicado: (2005)

Alternative Polyadenylation: Methods, Findings, and Impacts
por: Chen, Wei, et al.
Publicado: (2017)

Editorial: Alternative Polyadenylation in Development and Disease
por: Pai, Athma A., et al.
Publicado: (2022)

Regulation of alternative splicing and polyadenylation in neurons
por: Lee, Seungjae, et al.
Publicado: (2023)

Biochemical approach for isolation of polyadenylated RNAs with bound proteins from yeast
por: Matia-González, Ana M., et al.
Publicado: (2021)

Alternative polyadenylation mediates genetic regulation of gene expression
por: Mittleman, Briana E, et al.
Publicado: (2020)

Comprehensive mapping of alternative polyadenylation site usage and its dynamics at single-cell resolution
por: Wang, Junliang, et al.
Publicado: (2022)

An in-depth map of polyadenylation sites in cancer
por: Lin, Yuefeng, et al.
Publicado: (2012)

Conservation of alternative polyadenylation patterns in mammalian genes
por: Ara, Takeshi, et al.
Publicado: (2006)

Transcriptional activity regulates alternative cleavage and polyadenylation
por: Ji, Zhe, et al.
Publicado: (2011)

Extensive alternative polyadenylation during zebrafish development
por: Ulitsky, Igor, et al.
Publicado: (2012)

Genome-wide dynamics of alternative polyadenylation in rice
por: Fu, Haihui, et al.
Publicado: (2016)

Comparative analysis of alternative polyadenylation in S. cerevisiae and S. pombe
por: Liu, Xiaochuan, et al.
Publicado: (2017)

Dysfunctional transcripts are formed by alternative polyadenylation in OPMD
por: Raz, Vered, et al.
Publicado: (2017)

Template-switching artifacts resemble alternative polyadenylation
por: Balázs, Zsolt, et al.
Publicado: (2019)

A comprehensive map of alternative polyadenylation in African American and European American lung cancer patients
por: Zingone, Adriana, et al.
Publicado: (2021)

Spatial mapping of the total transcriptome by in situ polyadenylation
por: McKellar, David W., et al.
Publicado: (2022)

THOC5 controls 3′end-processing of immediate early genes via interaction with polyadenylation specific factor 100 (CPSF100)
por: Tran, Doan Duy Hai, et al.
Publicado: (2014)

UTR extension and alternate polyadenylation in neuroplasticity: an emerging paradigm?
por: Harrison, Benjamin J, et al.
Publicado: (2014)

Cell Cycle Regulation by Alternative Polyadenylation of CCND1
por: Wang, Qiong, et al.
Publicado: (2018)

Alternative polyadenylation factors link cell cycle to migration
por: Mitra, Mithun, et al.
Publicado: (2018)

The role of alternative polyadenylation in the antiviral innate immune response
por: Jia, Xin, et al.
Publicado: (2017)

The role of alternative Polyadenylation in regulation of rhythmic gene expression
por: Ptitsyna, Natalia, et al.
Publicado: (2017)

The transcriptional elongation rate regulates alternative polyadenylation in yeast
por: Geisberg, Joseph V, et al.
Publicado: (2020)

Extensive Involvement of Alternative Polyadenylation in Single-Nucleus Neurons
por: Wang, Ying, et al.
Publicado: (2020)

APAatlas: decoding alternative polyadenylation across human tissues
por: Hong, Wei, et al.
Publicado: (2020)

Alternative polyadenylation associated with prognosis and therapy in colorectal cancer
por: Zhang, Yi, et al.
Publicado: (2022)

The Role of Alternative Polyadenylation in the Regulation of Subcellular RNA Localization
por: Arora, Ankita, et al.
Publicado: (2022)

Alternative polyadenylation: methods, mechanism, function, and role in cancer
por: Zhang, Yi, et al.
Publicado: (2021)

Alternative polyadenylation of mRNA and its role in cancer
por: Yuan, Fuwen, et al.
Publicado: (2019)

Alternative polyadenylation is a determinant of oncogenic Ras function
por: Subramanian, Aishwarya, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_dmfk4kpb5g3mh4c5l86c5t2mli